"Joanna of Austria (in Castilian, doña Juana de Austria; in Portuguese, Dona Joana de Áustria, 24 June 1535 – 7 September 1573) was Princess of Portugal ...
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by marriage to John Manuel, Prince of Portugal. She served as regent of Spain to her brother Philip II of Spain during his trip to England to marry Mary I in 1554-1556, and from 1556 to 1559. She was the mother of King Sebastian of Portugal." - (en.wikipedia.org 03.10.2020)